E4S Enzyme
GMP grade collagenase
GMP Grade E4zyme AS is an aseptically filled, lyophilized mixture of 60% purified class I (C1) and 40% purified class II (C2) collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa. CIzyme AS can be used for isolating adipose derived stromal cells from human lipoaspirate.
GMP Grade E4zyme AS is an aseptically filled, lyophilized mixture of 60% purified class I (C1) and 40% purified class II (C2) collagenase from Clostridium histolyticum pre-mixed with neutral protease from Bacillus polymyxa. CIzyme AS can be used for isolating adipose derived stromal cells from human lipoaspirate.
- Manufactured for superior lot-to-lot enzyme consistency for improved performance consistency
- Optimal enzyme activity (28-42 Wünsch Units) and neutral protease activity (220,000 – 340,000 NP units) per vial to dissociate approximately 100 grams of human adipose tissue.
- Low contaminating endotoxin levels (＜25 EU/OD)
- White lyophilized cake under vacuum in amber bottle sealed with butyl rubber stopper
- Stable for at least 2 years when stored at 2-8 ℃
- Lyophilized in a buffer containing calcium to ensure enzyme stability during initial reconstitution.
| Appearance | White lyophilized cake |
|---|---|
| Total Wünsch Activity | 30 – 40 Units/Vial |
| Total Neutral Protease Activity | 220,000 – 350,000 Units/Vial |
| Storage | 2-8°C |
| Shelf Life | 2 Years |
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